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首页 > 蛋白生物学 > 蛋白Marker > 多肽电泳Marker

彩虹预染超低分子量蛋白Marker(1.2-45KD)

彩虹预染超低分子量蛋白Marker(1.2-45KD)

产品编号:RTD6110

产品规格:10次

数量
价格 ¥500

彩虹预染超低分子量蛋白质Marker1.2-45kD

Rainbow Prestained Ultra Low Molecular Weight Protein Marker

 

产品编号及规格:

RTD6110   10T50μl)   500元

储存条件:

  -20保存,开封后有效期12个月

产品简介:

    彩虹预染超低分子量蛋白质Marker可以直接观察蛋白电泳及清晰判断Western Blotting的转膜效果。本产品包含3种多肽和3种低分子量蛋白质组成,分子量范围为1.2kD-45kD,分子量大小为  1.2kD4.6kD10kD16kD27kD45kD (注:蛋白大小已在18%Tricine-SDS-PAGE中用非预染蛋白Marker标定过。




● 使用说明:

第一次收到该产品,常温融化后,彻底混匀,离心快甩将溶液完全收集到管底

. 制胶:

I 配制分离胶

1.按照表一将不同体积的双蒸水、40% PAA19:1)、4×凝胶缓冲液(货号:GB010)和乙二醇加入到小烧杯中混合。

2.加入10%APSTEMED,立即混匀5-10秒,以使溶液充分混匀。

3.在凝胶模具中迅速灌入适量分离胶溶液,然后在分离胶溶液上轻轻覆盖一层1 cm的无水乙醇,使凝胶表面保持平整。

4.静置15-30分钟,待分离胶和乙醇层之间出现一个清晰的界面表示凝胶已聚合。

表一  (一块1 mm mini胶用量)

 

分离胶

浓缩胶

 

18T, 5%C /5 ml

5T, 3.3%C/2 ml

40% PAA19:1

2.25 ml

/

40% PAA (29:1)

/

0.25 ml

4×凝胶缓冲液

1.25 ml

0.5 ml

乙二醇(电泳级)

1.5 ml

/

ddH2O

/

1.25ml

10APS

50 μl

20 μl

TEMED

5 μl

2 μl


II 配制浓缩胶

去除覆盖在分离胶上的乙醇层,用滤纸将残留乙醇吸去。

1.按照表一将不同体积的双蒸水、40%PAA(29:1)和4×凝胶缓冲液加入到小烧杯中混合。

2.加入10%过硫酸铵和TEMED,立即混匀5-10秒,以使溶液充分混匀。

3.将梳子插入凝胶内,避免产生气泡。

4.静置30-60分钟装待凝胶聚合

. 电泳:

1. Marker样品,充分混匀后备用。不要加热处理。

2. 电泳前,将10×阳极缓冲液(Cat No:AB080)和10×阴极缓冲液(Cat No:CB010)用蒸馏水稀释成1×缓冲液备用。将电泳槽的外槽加入1×阳极缓冲液,内槽加入1×阴极缓冲液,轻柔拔出梳子,将Marker或蛋白样品(已经过2×Tricine上样缓冲液[Cat No:TP050]处理)加入点样孔,参考下表进行电泳,至每条带都清晰可见时停止电泳。整个电泳过程大约需要2-3个小时。

表二 多肽电泳条件

恒电压

150V

起始电流

60-75mA/板胶

结束电流

15-25mA/板胶

电泳时间

2.5-3小时

. 染色

根据常规实验步骤,用配方6和7(表三)进行染色和观察。如果使用配方6进行染色时效果不好或考虑其毒性,请选择本公司的FastBlue蛋白染色液(货号:RTD6202),该产品除具有染色快,无毒,灵敏性高等特点外,还能对小肽在染色中起到固定作用,不至于小肽在染色和脱色中从凝胶中脱离,是常规染色液的理想替代品。


相关产品:


多肽电泳配套试剂

名称

货号

规格

超低分子量蛋白电泳试剂盒

RTD6120

10

超低分子量蛋白Marker I3.3-20.1 kD

RTD6101

10次(100 μl

超低分子量蛋白Marker III3.4-100 kD

RTD6125

10次(50 μl

彩虹预染超低分子量蛋白Marker1.2-45 kD

RTD6110

10 50 μl

彩虹预染超低分子量蛋白Marker II3-40 kD

RTD6145-01

10 50 μl

40% PAA19:1

AC1914-01

100ml

40% PAA29:1

AC2914-01

100ml

多肽电泳凝胶缓冲液

GB010-100ml

100ml

2×Tricine 上样缓冲液(变性,还原)

TP050

10×1ml

FastBlue蛋白染色液

RTD6202

500ml

乙二醇(电泳级)

EA0582-02

100 ml

10×Tris-Tricine-SDS缓冲液(变性电泳,粉末型)

CB010P

500 ml

10×阳极缓冲液 (多肽电泳用

AB080

250 ml

10×阴极缓冲液(多肽电泳用)

CB010

100 ml

 


使用RTD6110 彩虹预染超低分子量蛋白Marker1.2-45KD产品发表部分文章列表:

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieae isolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control 50 (2015) 896e900

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper linkhttp://dx.doi.org/10.1016/j.foodcont.2014.10.040

 

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control  Volume 81, November 2017, Pages 211-217

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper linkhttps://doi.org/10.1016/j.foodcont.2017.05.030

 

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper linkhttps://doi.org/10.1093/jxb/ery351

 

4. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

InstitutionInstitute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper linkhttps://doi.org/10.1093/jxb/ery351

 

5. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

InstitutionBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper linkhttps://doi.org/10.1093/plcell/koab046

 

6. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

Journal: Analytical Methods. 2021,13, 2804-2811

InstitutionDepartment of Chemistry, Fudan University

Paper linkhttps://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

 

7. [2022 IF=6] Peptide LQLY3-1, a novel Vibrio harveyi quorum sensing inhibitor produced by Lactococcus lactis LY3-1.

Author: Yangrui Wang, Mengtong Sun, Xiaoling Cui, Yongyue Gao, Xinran Lv, Jianrong Li, Fengling Bai, Xuepeng Li, Defu Zhang, Kai Zhou

Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

Journal: LWT - Food Science and Technology 170 (2022) 114093

InstitutionCollege of Food Science and Engineering, Bohai University

Paper linkhttps://doi.org/10.1016/j.lwt.2022.114093

 


 
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