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首页 > 核酸生物学 > 核酸提取 > RNaseA溶液

RNaseA溶液 10mg/ml

RNaseA溶液 10mg/ml

产品编号:RN001-02

产品规格:10×1ml

数量
价格 ¥500

RNaseA Solution  10mg/ml

RNaseA溶液

货号

规格

价格

备注




 

RN001-02

10×1ml

500

 

RN001-03

10×300 μl

150

配套质粒小提100次用

RN001-04

10×600 μl

300

配套质粒小提 200次用

 

 

 

 


● Introduction:

Ribonuclease A (RNase A) is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B it is not a glycoprotein. RNase A can be inhibited by alkylation of the histidine-12 or histidine-119 which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts. The optimal temperature for activity is60°C, although the enzyme does exhibit activity from 15-70°C. The pH optimum is 7.6, with an activity range of 6-10.  The highest activity is exhibited with single stranded RNA. RNase A is a very stable enzyme and can withstand temperatures up to100°C.  At100°C, RNase A is most stable between pH 2.0 and 4.5.

Stability:

RNase A power is recommended to be stored in a freezer location (-20°C). Stock solutions stored in frozen aliquots are stable for at least 6 months.

质粒提取中RNaseA的使用:

RNaseA在质粒中的终浓度推荐为100μg/ml,即在质粒溶液中按照1/100体积加入10mg/ml RNaseA即可,如50μl质粒中加入0.5μl RNaseA溶液,37℃孵育10分钟。


 
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