D2000 DNA ladder

● 产品简介：

本产品是由6条双链DNA条带组成，适用于琼脂糖凝胶电泳中DNA条带的分析。产品中已含有1×loading buffer，可根据实验需要，直接取2-5 μl电泳，使用方便，电泳图像清晰。6条带的大小分别为100，250，500，750，1000，2000 bp。其中750 bp条带浓度约为100 ng/5 μl，显示加亮带，其余条带浓度约为50 ng/ 5μl。

● 贮存、效期及运输：

-20℃贮存；有效期3年；湿冰运输。

● 使用方法：

1. 取2-5 μl本产品加入到琼脂糖凝胶的加样孔中（每1 mm×1 mm加样孔加1 μl，如果加样孔

宽于5 mm，可以适当增加上样量）就行电泳。

2. 建议电泳条件：凝胶浓度为1.0-2.0％，凝胶长度5-7 cm，电泳电压4-10 v/cm，电泳时间15-20分钟。

3. 通过核酸染料染色后紫外灯或蓝光仪观察条带。

● 注意事项：

1. 琼脂糖的质量对DNA的电泳有很大影响，电泳时请尽量使用质量优等的琼脂糖。

2. 由于PAGE电泳的特殊性，该产品不建议用于PAGE电泳。

3. 请使用新鲜配制的电泳缓冲液和新鲜配制的凝胶进行电泳，以保证Marker良好的分离效果。

4. 该Marker适用于DNA片段大小的确定和对DNA含量的粗略定量，不适用于DNA片段含量的精确定量。

5．推荐使用RealSafe系列染料（货号：GR001，GR002，GR003，GR004）染色。如果使用Goldview（货号：GV001）或EB（货号：EB001）等染料进行染色，由于此类染料带更多的正电荷（染料移动方向与核酸泳动方向相反），随着电泳时间延长，染料向大片段聚集，凝胶会出现阴阳胶现象（染料含量高的凝胶紫外下较亮，含量低的凝胶紫外下发暗），导致小分子

量DNA显色很弱或不可见。阴阳胶可以在电泳结束后浸泡染色，将胶浸没在含EB或Goldview的电泳缓冲液中摇动染色20-25 min，小分子量DNA会显色清楚。更长时间浸泡会因DNA分子扩散而使条带弥散，小分子量DNA更易弥散。

6．紫外照射下EB易分解，使DNA条带荧光变浅。DNA受紫外光照射形成嘧啶二聚体，不利于待回收片段的下游工作，因此尽可能减少紫外照射时间。

7．蔗糖会结合DNA，降低其电泳迁移率，建议DNA样品上样使用含甘油的上样缓冲液，如6×DNA上样缓冲液（双染料）（货号：DL020）。

8．大部分核酸工具酶会结合DNA，降低其电泳迁移率，大分子量DNA尤为明显，建议此类DNA样品上样使用含SDS的上样缓冲液，如6×DNA上样缓冲液（含SDS）（货号：DL090）。

使用RTM415  D2000 DNA ladder产品发表部分文章列表：

1. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

2. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

3. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

4. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

5. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

6. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

7. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5

8. [2022 IF=8.4] Target and acidic tumor microenvironment-induced in situ HCR for bispecific tumor cell imaging and therapy.

Marker :RTM415，D2000 DNA ladder

Author: Zihan Song, Yuzhe Fan, Su Zeng, Lianli Sun

Journal: Sensors and Actuators: B. Chemical  413 (2024) 135867

Institution：College of Pharmaceutical Sciences, Zhejiang University

Paper link：https://doi.org/10.1016/j.snb.2024.135867

9. [2023 IF=14.3] Bioactive Self-Assembled Nanoregulator Enhances Hematoma Resolution and Inhibits Neuroin?ammation in the Treatment of Intracerebral Hemorrhage.

Marker :RTM415，D2000 DNA ladder

Author: Wenyan Yu, Chengyuan Che, Yi Yang, Yuzhen Zhao, Junjie Liu, Aibing Chen,and Jinjin Shi

Journal: Adv. Sci. 2024, 2408647

Institution：School of Pharmaceutical Sciences，Zhengzhou University

Paper link：https://doi.org/10.1002/advs.202408647