DMSO-PCR级
Dimethyl Sulfoxide Solution-PCR Grade
● 产品货号及规格:
货号
|
名称
|
规格
|
贮存
|
RTE3102-02
|
DMSO溶液 PCR级
|
5×1 ml
|
-20℃
|
● 产品简介:
Betaine, DMSO and formamide helps in reducing the secondary
structure of GC rich templates and assists amplification of these templates.
Betaine is used at3.5Mto0.1M, DMSO should be used between 2-8%,
however 10% DMSO can reduce Taq polymerase activity by up to 50%. Formamide is
generally used at 1-5%. It has been our experience that the use of DMSO in
combination with betaine is superior to using formamide.
● 贮存:-20℃贮存。有效期二年。
● 使用说明:
1.-20℃保存的DMSO常温融化,混匀。
2. 根据根据PCR反应体系,加入适量体积的DMSO溶液。
|
20 μl 体系
|
反应体系终浓度
|
10×PCR Buffer
|
2 μl
|
1×
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DMSO*
|
0.4-1.6 μl
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2-8%
|
模板
|
0.05-0.5μg
|
as you wish
|
上游引物 10μM
|
0.5 μl
|
0.2 μM
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下游引物 10μM
|
0.5 μl
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0.2 μM
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dNTP10mM
|
0.5 μl
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200 μM
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Taq
|
0.5-1 μl
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2.5-5 U
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灭菌水
|
up to 20 μl
|
|
注*:不同模板,不同片段的扩增可能需要不同浓度的DMSO,可以适当调节加入体积,找到适合自己反应的甜菜碱浓度。
● References:
Frackman, S., Kobs,
G., Simpson, D. and Storts, D. 1998. Betaine and DMSO: enhancing agents for
PCR.
Promega
Notes 65: 27.